The provided transcript is in Indonesian and describes the procedure for a pharmacology practicum focusing on the absorption, distribution, and excretion (ADE) of a drug. The summary below is translated and structured according to the requirements.

Pharmacology Practicum Objectives and Subjects
📌 The practicum aims to understand the absorption, distribution, and excretion (ADE) of a drug after it enters the body.
🧑‍🤝‍🧑 Six healthy volunteers (male and female, aged 20-40) participated after providing written informed consent.
🔬 Subjects were screened for a history of liver, kidney, or stomach diseases and had no history of drug allergies.

Materials and Experimental Procedure
🧪 The key drug used was 0.3 g KI (Potassium Iodide) administered orally with 200 ml of water.
💧 Baseline samples, 5 ml of urine and 2 ml of saliva, were collected from each subject before drug administration as controls.
⏱️ Saliva samples were collected at 5-minute intervals up to 90 minutes, while urine samples were collected at 15-minute intervals up to 90 minutes post-administration.

Analysis and Data Compilation
🌈 The concentration of iodide ($I^-$) in urine and saliva was measured using semi-quantitative colorimetry based on color gradation changes compared to a positive control.
📈 The darkest color indicates the highest positive value, with the maximum grading being positive 3 (a semi-quantitative data point).
📊 Final data must be tabulated, and a time versus iodine concentration curve for both urine and saliva must be generated for the final lab report.

Key Points & Insights
➡️ The experiment directly models the body's processes for how a substance is absorbed, distributed, and eliminated by monitoring iodine levels in bodily fluids.
➡️ Informed consent and subject screening are crucial preparatory steps to ensure data validity and subject safety in pharmacological studies.
➡️ The use of colorimetry for semi-quantitative analysis provides a practical, albeit comparative, method to track drug elimination kinetics.

📸 Video summarized with SummaryTube.com on Oct 10, 2025, 07:15 UTC