Extraction Procedure: Fractionation using Ethyl Acetate
📌 The practical session involves introducing green algae extract dissolved in ethanol into the process.
⚗️ Fractionation is performed using a solvent with different polarity, specifically ethyl acetate (a less polar solvent).
🌡️ After mixing, the solution is allowed to stand upright to facilitate phase separation between ethanol and ethyl acetate.
💨 The final step involves evaporating the collected fractions on a water bath or, more safely, using a rotary evaporator to obtain concentrated extracts.

Chemical Separation Technique
🧪 The goal of using ethyl acetate is to separate compounds that are soluble in ethyl acetate from those that are not, based on polarity differences.
💧 After separation, two distinct phases (upper and lower) are formed, requiring careful identification of the ethyl acetate phase and the ethanol phase.
⚠️ Care must be taken during the separation of the two phases to ensure the separation runs perfectly without mixing.

Key Points & Insights
➡️ To achieve phase separation, the container must be in a standing, upright position to allow the difference in density and polarity to work effectively.
➡️ Always vent the system slightly (opening the container cap) to prevent excessive pressure buildup during the process.
➡️ Fractions collected from the separation process must be evaporated separately to obtain their respective concentrated extracts.

📸 Video summarized with SummaryTube.com on Dec 28, 2025, 08:19 UTC