Pharmacology Practical: Drug Administration Routes
📌 The objective of this practical pharmacology session is to observe and compare the effects of a drug (Diazepam) administered via different routes in rats.
🐭 The subjects used are rats, and the tools include a 1 mL syringe, stomach tube, rat cages, and a fixator.
🧪 The drug being tested is Diazepam (5 mg/mL solution or tablet form).

Experimental Procedure Setup
🧑‍🔬 Students were divided into five groups, with each group working on four rats.
💉 Each rat was assigned a specific administration route: Rat A (Oral), Rat B (Intramuscular - IM), Rat C (Intravenous - IV), and Rat D (Intraperitoneal - IP).
🧼 Injection sites must be cleaned first with warm water and then with 70% alcohol before administration.

Observation and Data Collection
⏱️ Key observations include the onset time of sedation and the time until sleep after drug administration for each route.
🤸‍♀️ To assess sedation, rats are dropped from a height of 30 cm; a successful drop means landing on all four paws, indicating minimal sedative effect.
📝 Detailed reports must be compiled, recording the time interval between the drug administration, the onset of sedation, time to sleep, and time to wakefulness.

Key Points & Insights
➡️ The practice aims to highlight the differences in pharmacokinetics (absorption rate) based on the route of administration (e.g., IV is typically the fastest).
➡️ Diazepam is the chosen drug to measure sedative effects across Oral, IM, IV, and IP routes.
➡️ Careful observation and precise timing of sedation/sleep onset are critical for comparing the speed and efficacy of drug delivery methods.

📸 Video summarized with SummaryTube.com on Oct 10, 2025, 08:55 UTC